When an individual touches a surface, trace amounts of biological material are transferred and can be sampled to generate a DNA profile, which can be a crucial identification method in forensic investigations. This evidence is referred to as touch DNA. Studies on the recovery of touch DNA have been conducted to investigate the natural degradation over time, as a response to factors like temperature, humidity, and even collection methods. Nevertheless, there is a lack of research on the effect of the surface material itself. Therefore, this study aims to evaluate the persistence of touch DNA on glass, metal, and plastic over time to determine if touch DNA degrades at different rates across various surface types. Under controlled environmental conditions, touch DNA was placed by one individual on sterilized surfaces represented by glass, metal and plastic.. Each surface type will be swabbed in triplicate at five-day intervals over a 30-day period. Total genomic DNA will be extracted from all samples, followed by real-time quantitative polymerase chain reaction (qPCR), using a human specific primer assay to investigate persistence of human DNA. Statistical analyses will be used to determine if there are significant differences in DNA concentrations over time and if there are significant differences between glass, metal, and plastic. Results from this study will contribute to forensic investigations by providing deeper insight on which surfaces may be more productive to swab for touch DNA when investigators process a crime scene.