Air particulate matter (PM) consists of a mix of solid particles and liquid droplets suspended in air, including organic, inorganic, and biological materials. Interest in PM is largely due to the inhalable aspect of PM10 (<10 µm) and PM2.5 (<2.5 µm), which can travel deep into the human respiratory system, with PM2.5 capable of penetrating the lungs and entering our bloodstream, transporting toxins directly to vital organs. The chemical characterization of organic PM2.5 is incomplete and usually excludes pollen, and most other biogenic sources. Pollen falls in the size range between 10-100 µm in diameter, but heavy rain and thunderstorms cause pollen grains to rupture or fragment, releasing PM within the inhalable range. Traditionally characterized organics in PM2.5 are largely due to industrial emissions, vehicle emissions, and waste/forest burning, but the extent of pollen in air PM is not well understood. In this study, air PM2.5 samples were collected over two months through the peak of harvest outside of Grand Forks, ND. A diaphragm pump maintained flow through a cutpoint cyclone such that PM2.5 was collected on quartz filters. Sample punches of the quartz filters were used to correlate carbon content during different sampling periods using an organic carbon elemental carbon aerosol analyzer. Furthermore, DNA sequencing was performed on the filters to determine biological markers and identify species associated with the PM. Genetic material was identified from Bacteria, Archaea, and Eukarya, with the most common species including single-cell organisms, plant pollen, and members Archaea and Bacteria.