Forensic microbiology as it relates to touch microbiome is an emerging field in the world of forensic science and can be a potential tool for identifying and connecting an individual to a location or an item. Human skin microbiomes act like a biological fingerprint, being influenced by the location, environment, diet, everyday activities and hygiene etiquettes. In cases where DNA evidence is degraded or unavailable, this pilot study addresses the question of whether human microbiomes can connect individuals to objects and crime scenes by evaluating microbiome transfer and persistence over a long duration with repeated sampling. Three personal items; a phone, car steering wheel and TV remote as well and an untouched control object were sampled over a 14-week sampling period in an apartment setting. Total genomic DNA was extracted using a Qiagen microbial DNA extraction kit, and microbial abundance will be quantified using TaqMan-based Quantitative Polymerase Chain Reaction (qPCR) with TaqMan Fast Advanced Master Mix and Microbe Assay Single assays. The hypothesis is that microbiome abundance and persistence will vary among the three personal objects based on the frequency use and exposure to different environments. These findings will improve our understanding of human-to-surface microbial transfer and persistence under everyday conditions and help evaluate the forensic utility of microbiome evidence for human identification.