Cell migration relies on precise regulation of cellular adhesion to the extracellular matrix (ECM). Cell adhesion is mediated by focal adhesion complexes (FAs). FA engagement with the ECM both anchors the cell to its environment and permits mechanotransduction. Subsequent detachment of FAs allows cells to move according to generated forces. Coordination of FA dynamics throughout the plasma membrane dictates cell migration, and more rapid FA turnover facilitates increased migration and invasion. Dysregulation of cellular adhesion contributes to breast cancer progression and metastasis. Approximately one-third of breast cancer patients are diagnosed with depression, and breast cancer patients are prescribed selective serotonin reuptake inhibitors (SSRIs) more often than any other type of cancer patient. Despite these observations, the question of how SSRIs impact disease progression remains relatively unexplored. Broadly, SSRIs increase extracellular serotonin. Serotonin treatment enhances the metastatic potential of breast cancer cells by promoting migration and invasion. Considering that abnormal cell migration is the fundamental issue of breast cancer progression and metastasis, and that adhesion governs migration, we aim to investigate the role that serotonin plays in modulating FA dynamics. Quantitative immunofluorescence confocal microscopy has been paired with migration assays to characterize FA morphology and determine if singe-cell changes translate to altered collective cell behavior. We demonstrate that breast epithelial cell lines treated with serotonin display an increase in number of FAs as well as an increase in migratory capacity.