Biological evidence recovered from crime scenes may often be compromised by environmental conditions. Such is the case in aquatic environments, where water exposure is known to accelerate DNA degradation. In forensic laboratories, limited time and resources require examiners to prioritize evidence most likely to yield usable DNA profiles. Simulating a scenario in which items of clothing are recovered from a body of water, this study aims to aid forensic practitioners by providing data on touch DNA persistence on submerged fabric. DNA persistence on fabric will be tested in triplicate from freshwater and saltwater environments. A no water environment will be used as control. White cotton T-shirts, worn for 12 hours overnight were submerged in prepared 3-gallon water tanks for time periods ranging from 15 minutes to 1 month. DNA will be extracted from 0.5x0.5cm fabric sections collected at various predetermined time points and quantified. It is expected that DNA concentrations will decrease over time following submersion in both aquatic environments. Differences in degradation between saltwater and freshwater environments will also be investigated. Changes in DNA concentrations over time will be analyzed to determine a time in which it is no longer likely to yield a usable DNA sample from submerged fabrics.