Alzheimer's disease (AD) is an age-associated dementing disease that affects millions of people every year and is expected to rise with the increasing elderly population. Numerous neurotransmitter systems are altered in the brain during disease. Although AD is classically defined as a brain disease, clinical and experimental evidence suggest that AD affects the periphery as well, including the gastrointestinal system. In this study we asked whether the enteric nervous system of the intestines displays neurotransmitter dysfunction similar to changes observed in the central nervous system. We focused on the dopaminergic system based on its well-described deficits in AD and the critical role of dopamine in modulating intestinal physiology. We compared 12-month-old male and female littermate control wild type mice to an AD mouse model, AppNL-G-F, mice using immunohistochemistry and high-performance liquid chromatography. Total dopamine levels in the colons of both male and female AppNL-G-F mice were elevated but not significantly different from their respective wild type controls. Consistent with this observation, immunostaining for tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine production, showed increased male AppNL-G-F TH staining compared to their wild type controls. Female mice had no significant difference in TH staining compared to wild type mice. These findings suggest that disruptions of neurotransmitter systems, including dopamine, may not be limited to the brain in AD and these changes may be sex dependent.