Fluorescence nanocomposites have garnered considerable interest due to their high fluorescence intensity and photostability. Autofluorescence from biological samples in the UV visible range causes interference; thus, NIR fluorescence is more usable for bioimaging. In this work, Tetrakis(4-carboxyphenyl) porphyrin (TCPP) based hybrid nanoparticles incorporating phenylboronic acid (PBA) and 3-mercaptopropyltrimethoxysilane (MPTMS) were used for making NIR nanoparticles based on a hydrothermal synthesis approach. The self-assembly of TCPP under hydrothermal conditions facilitates the formation of a porphyrin-based nanostructure. Phenylboronic acid functionalization enhances stability and introduces potential targeting capabilities through boron. 3-mercaptopropyltrimethoxysilane can contribute to a protective silica based network, further enhancing nanoparticle robustness, dispersibility, and surface reactivity through its thiol functional groups. The Dynamic light scattering (DLS), transmission electron microscopy (TEM) imaging, UV-vis, and fluorescence studies were conducted to characterize and investigate the various properties of these nanoparticles. Results showed the nanoparticles have a diameter of 19.7 + 4.7nm. The UV-vis and fluorescence studies confirmed that the nanoparticles exhibited two fluorescence emission peaks at 647.3 nm and 703.2 nm. Further, the stability of the nanoparticles was verified through the Zeta potential, which was ≥ 30 mV or ≤ -30 mV when the pH of the nanoparticles in the range of 1 to 11. Applications of nanoparticles were carried out in cell imaging. The experimental results showed strong fluorescence signals when cells were labeled. RAW 264.7 cells were used for the bioimaging study, and the cells were clearly labeled with nanoparticles. These results highlight TCPP–PBA–MPTMS hybrid nanoparticles as promising NIR probes for bioimaging applications.